Jimofoz 1 Posted September 7, 2020 Share Posted September 7, 2020 What delivery vector are you planning to use to get the Draco inside every potentially infected cell? Have you heard of Oisin Biotechnologies and their “fusogenix” lipid nanoparticles? Link to post Share on other sites
Rick 34 Posted September 7, 2020 Share Posted September 7, 2020 The original DRACO research used three different transduction tags (also known as Cellular Penetrating Peptides), and found them to be effective at reaching every infected cell. We are planning to use that proven approach, at least at first. However, in the long term, alternative delivery mechanisms are an area we would very much like to explore. Fusogenix is an interesting one, and there are a few others on our radar, as well. Link to post Share on other sites
abotnick 0 Posted February 13 Share Posted February 13 What's the molecular weight of the VTose system? What is the largest molecular weight that can be encapsulated into a protein transduction tag? Immune reaction is a major side effect of the injected PTTs. Quote One of the current challenges of injectable PDs is their immune response, rendering them less effective or even causing the production of toxic inhibitory antibodies (immunoglobulin E (IgE)), resulting in anaphylaxis or death. Such inhibitory antibodies form in ~25–30% of patients with severe hemophilia A and in ~5% of severe hemophilia B patients. 126 Inhibitors seriously complicate clotting factor replacement therapy and thus increase morbidity and mortality of the disease. https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)33585-7 I'm not sure if this would be a problem with the oral form as well. Link to post Share on other sites
Rick 34 Posted February 13 Share Posted February 13 57 minutes ago, abotnick said: What's the molecular weight of the VTose system? What is the largest molecular weight that can be encapsulated into a protein transduction tag? The molecular weight of VTose is roughly 35 kDa. One of the earliest papers about transduction tags showed effectiveness with a protein with a MW of 120 kDa: In vivo protein transduction: delivery of a biologically active protein into the mouse - PubMed (nih.gov) Transport of even larger proteins is undoubtedly possible. 1 Link to post Share on other sites
abotnick 0 Posted February 19 Share Posted February 19 (edited) That's encouraging. A low inflammatory diet will minimize immune cytokine damage. Make GcMAF a recommended contraindication as it increases antibody production. If VTose works you shouldn't need it. ME patient antibody production is suppressed anyway by the viral nagalase so it may not end up being a problem. Critical Covid and Nutrition Information for Patients.pdf Edited February 19 by abotnick Link to post Share on other sites
abotnick 0 Posted February 26 Share Posted February 26 I caught this story on Oramed which delivers proteins to treat diabetes which are encapsulated and then have increased absorption in the small intesting. Perhaps it might be applicable for VTose. https://jewtube.tv/innovation/israeli-pharmaceutical-developed-the-first-pill-to-treat-diabetes-without-insulin-shots/ https://www.oramed.com/technology/scientific-abstracts/ Link to post Share on other sites
abotnick 0 Posted February 26 Share Posted February 26 22 hours ago, abotnick said: I caught this story on Oramed which delivers proteins to treat diabetes which are encapsulated and then have increased absorption in the small intesting. Perhaps it might be applicable for VTose. https://jewtube.tv/innovation/israeli-pharmaceutical-developed-the-first-pill-to-treat-diabetes-without-insulin-shots/ https://www.oramed.com/technology/scientific-abstracts/ @Rick Link to post Share on other sites
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