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The original DRACO research used three different transduction tags (also known as Cellular Penetrating Peptides), and found them to be effective at reaching every infected cell. We are planning to use that proven approach, at least at first.

However, in the long term, alternative delivery mechanisms are an area we would very much like to explore. Fusogenix is an interesting one, and there are a few others on our radar, as well.

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  • 5 months later...

What's the molecular weight of the VTose system? What is the largest molecular weight that can be encapsulated into a protein transduction tag?  Immune reaction is a major side effect of the injected PTTs.  

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One of the current challenges of injectable PDs is their immune response, rendering them less effective or even causing the production of toxic inhibitory antibodies (immunoglobulin E (IgE)), resulting in anaphylaxis or death. Such inhibitory antibodies form in ~25–30% of patients with severe hemophilia A and in ~5% of severe hemophilia B patients.

126

 Inhibitors seriously complicate clotting factor replacement therapy and thus increase morbidity and mortality of the disease.

https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)33585-7

I'm not sure if this would be a problem with the oral form as well.

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57 minutes ago, abotnick said:

What's the molecular weight of the VTose system? What is the largest molecular weight that can be encapsulated into a protein transduction tag? 

The molecular weight of VTose is roughly 35 kDa. One of the earliest papers about transduction tags showed effectiveness with a protein with a MW of 120 kDa:

In vivo protein transduction: delivery of a biologically active protein into the mouse - PubMed (nih.gov)

Transport of even larger proteins is undoubtedly possible.

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That's encouraging. A low inflammatory diet will minimize immune cytokine damage. Make GcMAF a recommended contraindication as it increases antibody production.  If VTose works you shouldn't need it.  ME patient antibody production is suppressed anyway by the viral nagalase so it may not end up being a problem.

Critical Covid and Nutrition Information for Patients.pdf

Edited by abotnick
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